Successful Formulation and Application of Low-Temperature Bacterial Agents for Corn Stover Degradation
نویسندگان
چکیده
Solid bacterial agents are required to accelerate stover degradation in low-temperature areas. However, the laboratory-to-practice translation of bioprocessing techniques is hindered by high cost, poor practicality, and short shelf life. Using corn powder, starch, bran as additives, we screened Pseudomonas putida Acinetobacter lwoffii, which effectively degrades at low temperatures, develop a sustainable low-cost agent formula that ensures viability soil storage. The optimal formulation included precipitates additives 1:4 ratio, including 4:3:9 ratio. viable count with this reached 7.5 × 1010 colony-forming units/g, lignocellulase activities. effect on its components, both lab culture simulation field environment, was significantly higher than without application. This had an outstanding lignin. storage conditions vacuum packing under 10% water content 4 °C; survival rate bacteria 85.33% after 180 d. Given global value stover-return agriculture, our results offer valuable strategy for application soils where rates otherwise low.
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ژورنال
عنوان ژورنال: Agronomy
سال: 2023
ISSN: ['2156-3276', '0065-4663']
DOI: https://doi.org/10.3390/agronomy13041032